LIUJing-miao,BIXue-jun,YANG Xin-ci,et al.Nitrogen Removal Performance of a Pilot?scale Pure Moving Bed Biofilm Reactor System at Low Temperature[J].China Water & Wastewater,2023,39(19):19-26.
低温条件下纯膜MBBR系统脱氮能力中试研究
- Title:
- Nitrogen Removal Performance of a Pilot?scale Pure Moving Bed Biofilm Reactor System at Low Temperature
- Keywords:
- moving bed biofilm reactor; low temperature; nitrogen removal; active biomass; microbial community structure
- 摘要:
- 针对传统活性污泥低温硝化限制性瓶颈问题，构建了两段式A/O耦合纯膜MBBR系统，开展了低温条件下纯膜MBBR系统生物脱氮能力中试研究，并利用分子生物学技术分析了纯膜MBBR系统内微生物群落结构变化对生物脱氮作用的影响机制。结果表明，在反应温度为10~12 ℃、生物膜载体在缺氧池和好氧池的填充率分别为40%和50%、系统进水有机物与氨氮容积负荷（以SCOD和NH4+-N计）分别为（236.94±42.63） g/（m3·d）与（56.93±2.97） g/（m3·d）的条件下，系统对SCOD、NH4+-N、总无机氮（TIN）的去除率分别为（71.20±4.64）%、（93.26±2.70）%、（62.30±2.41）%，出水浓度分别稳定在（45.29±4.42）、（2.56±1.02）、（14.92±1.20） mg/L；由于可利用碳源性质与结构的不同，导致前置与后置缺氧池在反硝化微生物群落结构方面存在显著差异；各好氧池形成明显的功能区化及其与之相适应的生物膜结构，生物膜载体表面最大硝化能力（以NH4+-N计）在0.49~1.07 g/（m2·d）之间，各好氧池的硝化能力与自养活性生物量和硝化菌相对丰度呈正相关。
- Abstract:
- This study constructed a pilot?scale two-stage A/O coupled with pure moving bed biofilm reactor (MBBR) system to investigate its biological nitrogen removal performance at low temperature, and analyzed the effect of microbial community structure change on the biological nitrogen removal through molecular biology techniques, so as to solve the bottleneck problem of nitrification of traditional activated sludge at low temperature. When the reaction temperature was 10-12 ℃, the packing rates of the media in the anoxic and aerobic tanks were 40% and 50% respectively, and the volumetric loads of organic matter (SCOD) and ammonia nitrogen were (236.94±42.63) g/(m3·d) and (56.93±2.97) g/(m3·d), respectively, the removal rates of SCOD, NH4+-N and total inorganic nitrogen (TIN) were (71.20±4.64)%, (93.26±2.70)% and (62.30±2.41)%, and their concentrations in the effluent stabilized at (45.29±4.42) mg/L, (2.56±1.02) mg/L and (14.92±1.20) mg/L, respectively. The different properties and structures of available carbon sources led to significant differences in the denitrifying microbial community structure between the pre-anoxic reactor and post?anoxic reactor. Each of the aerobic reactors formed an obvious functional zone and corresponding biofilm structure. The maximum nitrification rate (measured by NH4+-N) on the surface of the packing media was 0.49-1.07 g/(m2·d), and the nitrification rate of each aerobic tank was positively correlated with the autotrophic active biomass and the relative abundance of nitrifying bacteria.
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