[1]雷旭阳,张志华,崔广宇,等.蚯蚓对城镇污泥堆肥过程稳定化路径的影响[J].中国给水排水,2023,39(21):13-20.
　LEIXu-yang,ZHANG Zhi-hua,CUIGuang-yu,et al.Effect of Earthworms on Stabilization Path of the Composting Process for Municipal Sludge[J].China Water & Wastewater,2023,39(21):13-20.
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蚯蚓对城镇污泥堆肥过程稳定化路径的影响

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第39卷 期数: 2023年第21期 页码: 13-20 栏目: 出版日期: 2023-11-01
Title:
Effect of Earthworms on Stabilization Path of the Composting Process for Municipal Sludge
作者:
雷旭阳1， 张志华1， 崔广宇2， 刘艳娟3， 侯素霞1， 李博1
（1.河北科技工程职业技术大学 资源与环境工程系，河北 邢台 054000；2.北京大学深圳研究生院 环境与能源学院，广东 深圳 518055；3.唐山学院 新材料与化学工程学院，河北 唐山 063000）
Author(s):
LEI　Xu-yang1， ZHANG Zhi-hua1， CUI　Guang-yu2， LIU　Yan-juan3， HOU　Su-xia1， LI　Bo1
（1. Department of Resource and Environmental Engineering， Hebei Vocational University of Technology and Engineering， Xingtai 054000， China； 2. School of Environment and Energy， Peking University Shenzhen Graduate School， Shenzhen 518055， China； 3. Schcool of New Materials and Chemical Engineering， Tangshan University， Tangshan 063000， China）
关键词:
蚯蚓;  城镇污泥;  堆肥;  稳定化路径;  PCR-DGGE
Keywords:
earthworm;  municipal sludge;  composting;  stabilization path;  PCR-DGGE
摘要:
在20 ℃条件下，利用蚯蚓（Eisenia foetida）堆肥处理5 mm粒径城镇污泥，通过考察宏观理化指标的变化，研究了蚯蚓对城镇污泥堆肥过程稳定化路径的影响，并采用PCR-DGGE技术探究了微生物种群结构的变化。理化指标结果显示，随着堆肥时间的延长，蚯蚓处理组和对照组的有机质（OM）、DOC、NH4+-N变化趋势基本一致。其中，OM均显著降低、DOC先升高后降低、NH4+-N均显著升高，对照组的NO3--N含量始终维持在较低水平，蚯蚓处理组的NO3--N含量在50 d后出现激增。堆肥结束时，蚯蚓处理组的OM、DOC显著低于对照组（p<0.05），NH4+-N和NO3--N含量显著高于对照组（p<0.05）。主成分分析（PCA）结果表明：蚯蚓处理组和对照组稳定化过程均需依次经历有机质降解、溶解性有机碳积累、氨化和硝化为特征的反应历程。蚯蚓仅提高了城镇污泥的稳定化速率，但并未改变其稳定化路径。PCR-DGGE结果显示：堆肥结束时，蚯蚓处理组和对照组均以分解木质素、纤维素、几丁质等难降解有机质的微生物种群为主，并且蚯蚓增强了系统中被孢霉目（Mortierellales）微生物的相对丰度，能够削减堆肥产物中的植物致病菌。蚯蚓通过改变系统环境，进而促进系统微生物种群的更替，但并未颠覆系统的微生物种群结构。
Abstract:
At 20 ℃, Eisenia foetida was used to treat municipal sludge with a particle size of 5 mm. The influence of earthworms on the stabilization path of municipal sludge composting process was studied by examining the changes in physicochemical indicators, and the changes in microbial population structure were explored using PCR-DGGE technology. The results indicated that the change trend of both the earthworm treatment group and control group were essentially identical. As composting time increased, organic matter (OM) decreased significantly, dissolved organic carbon (DOC) initially increased and then decreased, NH4+-N increased significantly, NO3--N in the control group remained low while it sharply rose after 50 days in the earthworm treatment group. At the end of composting, OM and DOC were considerably lower in the earthworm treatment group than those in the control group (p<0.05), whereas NH4+-N and NO3--N were notably higher than those in the control group (p<0.05). Principal component analysis (PCA) revealed that both groups’ stabilization process was characterized by degradation of organic matter, accumulation of DOC, ammonification and nitrification in order. Earthworms only accelerated municipal sludge’s stabilization rate but did not alter its stabilization path. The results of PCR-DGGE analysis revealed that both the earthworm treatment group and the control group exhibited a dominance of microorganisms involved in lignin, cellulose, chitin, and other refractory organic matter at the end of composting. Additionally, there was an enhanced relative abundance of Mortierellales within the system which contributed to a reduction in plant pathogens present in the compost products. Earthworms facilitate changes in the environmental conditions promoting microbial population turnover without disrupting its overall structure.
更新日期/Last Update: 2023-11-01