[1]唐欣,李彦澄,钟雄,等.基于酿酒废水碳源的脱氮效能及微生物作用机理[J].中国给水排水,2024,40(9):56-62.
　TANGXin,LIYan-cheng,ZHONGXiong,et al.Nitrogen Removal Performance and Microbial Degradation Mechanism of Carbon Source from Brewing Wastewater[J].China Water & Wastewater,2024,40(9):56-62.
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基于酿酒废水碳源的脱氮效能及微生物作用机理

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第40卷 期数: 2024年第9期 页码: 56-62 栏目: 出版日期: 2024-05-01
Title:
Nitrogen Removal Performance and Microbial Degradation Mechanism of Carbon Source from Brewing Wastewater
作者:
唐欣1， 李彦澄1,2， 钟雄1， 陈晨1， 李江1,2， 杨爱江1,2
（1.贵州大学资源与环境工程学院 喀斯特地质资源与环境教育部重点实验室，贵州 贵阳 550025；2.贵州喀斯特环境生态系统教育部野外科学观测研究站，贵州 贵阳 550025）
Author(s):
TANG　Xin1， LI　Yan-cheng1，2， ZHONG　Xiong1， CHEN　Chen1， LI　Jiang1，2， YANG　Ai-jiang1，2
（1. Key Laboratory of Karst Geological Resources and Environment ， College of Resources and Environmental Engineering， Guizhou University， Guiyang 550025， China； 2. Guizhou Karst Environmental Ecosystems Observation and Research Station ， Guiyang 550025， China）
关键词:
酿酒废水;  液体碳源;  生物脱氮;  宏基因组;  代谢路径
Keywords:
brewing wastewater;  liquid carbon source;  biological nitrogen removal;  metagenome;  metabolic pathway
摘要:
针对低C/N生活污水，采用高浓度酿酒废水作为液体碳源，构建反硝化脱氮生物处理系统，研究容积负荷、溶解氧和停曝比对脱氮效能的影响，并通过三维荧光光谱、扫描电子显微镜和宏基因组分析系统的微生物作用机理。结果表明，系统的最佳容积负荷为0.111 kgCOD/（m3·d）、溶解氧为1~2 mg/L、停曝比为3∶1；通过三维荧光光谱分析发现，系统对类溶解性微生物代谢产物的去除效果较为明显，对类色氨酸蛋白质物质、类富里酸物质、类黑精物和类木质纤维素物质等有一定的降解；采用扫描电子显微镜发现，微生物主要为球状、杆状、丝状和螺旋状；通过宏基因组技术发现系统中的优势属分别为微白霜菌属（Micropruina）、丙酸菌属（Propionibacterium）、中村氏菌属（Nakamurella）。基于KEGG数据库功能注释结果显示，代谢功能占主导地位，系统中与氮代谢相关的完整路径有6条，其中以反硝化功能为主；与碳代谢相关的完整路径有47条，其中以三羧酸（TCA）循环为主。
Abstract:
This paper constructed a biological denitrification system for treating low C/N ratio domestic sewage with high-concentration brewing wastewater as liquid carbon source, investigated the effects of volumetric loading rate, dissolved oxygen and air pump on/off ratio on nitrogen removal efficiency, and analyzed the microbial degradation mechanism of the system by three-dimensional fluorescence spectrum, scanning electron microscopy and metagenomics. The optimal operational parameters were as follows: the volumetric loading rate was 0.111 kgCOD/(m3·d), the dissolved oxygen was 1-2 mg/L, and the air pump on/off ratio was 3∶1. The three-dimensional fluorescence spectrum analysis showed that the system had obvious effect on the removal of dissolved microbial metabolites, and had certain degradation ability of tryptophan-like protein substances, fulvic acid-like substances, melanoid-like substances and lignocellulose-like substances. The scanning electron microscopy results showed that the shape of microorganisms was mainly spherical, rod-shaped, filamentous and helical. The dominant genera in the system were Micropruina, Propionibacterium and Nakamurella as analyzed by metagenomic technology. The annotation results based on KEGG database showed that the dominant function was metabolism, and there were 6 complete pathways related to nitrogen metabolism in the system, among which denitrification was the main pathway. In addition, there were 47 complete pathways related to carbon metabolism, among which tricarboxylic acid (TCA) cycle was the main one.

相似文献/References:

[1]王洋,黄奇云,谢杰,等.农村排水户酿酒废水收集、预处理和集中处理[J].中国给水排水,2022,38(22):123.
　WANGYang,HUANGQi-yun,XIE Jie,et al.Collection, Pretreatment and Centralized Treatment of Brewing Wastewater in Rural Drainage Households[J].China Water & Wastewater,2022,38(9):123.

更新日期/Last Update: 2024-05-01