LIUQi-xin,FENGXuan,CHENNing,et al.Micro-response Characteristics of Aerobic Granular Sludge under Stress of Ribavirin[J].China Water & Wastewater,2024,40(23):29-39.
利巴韦林胁迫下好氧颗粒污泥的微观响应特征
- Title:
- Micro-response Characteristics of Aerobic Granular Sludge under Stress of Ribavirin
- Keywords:
- aerobic granular sludge; ribavirin; extracellular polymeric substances; signal molecule; microbial community composition
- 摘要:
- 新型冠状病毒肺炎（COVID-19）的暴发导致大量新冠药物被使用，部分药物及其代谢产物随下水道排入污水处理厂，目前已在污水处理厂及自然水体中检测到了抗病毒药物利巴韦林的存在。为此，通过考察在利巴韦林胁迫下好氧颗粒污泥（AGS）对其去除效果，以及AGS为应对利巴韦林胁迫而做出的微观响应特征，探究AGS形成过程的调控因子和维稳因素。结果表明，AGS在形成过程中对利巴韦林的去除率不断升高，最终稳定达到99%以上，并以生物降解为主。紧密结合型蛋白质（TB-PN）是在有或无利巴韦林胁迫条件下胞外聚合物（EPS）中均有且重要的组分，利巴韦林胁迫会刺激AGS培养前期微生物分泌较多EPS，表明AGS对于利巴韦林胁迫具有应激保护反应，且过多分泌的EPS会提高功能微生物间的电子传递效率，从而提高污染物降解效率。常规培养条件下，AHLs中的信号分子C8-HSL与EPS中蛋白质和多糖含量呈正相关，C8-HSL是促进TB-PN合成的关键信号分子。利巴韦林胁迫下C8-HSL更多负责指导多糖类物质的合成，表明利巴韦林可能干扰或抑制了TB-PN合成的通路，C4-HSL相关性分析同样证明利巴韦林的胁迫导致污泥微生物的代谢途径发生了变化。利巴韦林胁迫对微生物群落结构具有较强的干扰作用，在AGS培养前期促进了信号分子合成菌Acidovorax、耐药菌Acinetobacter及Delftia等可分泌生物毒性因子的菌属的优势生长，但不同条件下AGS系统中微生物群落组成最终达到高度相似，表明微生物多样性和群落演替是AGS保持稳定及良好污染物去除能力的主要原因。
- Abstract:
- The emergence of corona virus disease (COVID-19) has resulted in the widespread utilization of numerous antiviral medications, leading to the discharge of certain drugs and their metabolites into wastewater treatment plants (WWTPs) through sanitary sewers. Notably, the presence of antiviral drug ribavirin has been identified in both WWTPs and natural water bodies. Consequently, this paper investigated the removal efficiency of aerobic granular sludge (AGS) under ribavirin stress and examined the microscopic response characteristics of AGS to such stress, thereby elucidating the regulatory factors and stability maintenance mechanisms involved in the formation of AGS. Throughout the development of AGS, the ribavirin removal efficiency exhibited a continuous increase, ultimately stabilizing at over 99%, and the removal was primarily through biodegradation. Tightly bound protein (TB-PN) served as a crucial component of extracellular polymeric substances (EPS), both in the presence and absence of ribavirin stress. Ribavirin stress showed to enhance EPS secretion by microorganisms during the early stage of AGS culture, suggesting that AGS exhibited a protective response to ribavirin-induced stress. Furthermore, an excessive secretion of EPS augmented electron transfer efficiency among functional microorganisms, thereby enhancing the pollutants degradation efficacy. Under conventional culture conditions, the concentration of C8-HSL in AHLs exhibited a positive correlation with the contents of protein and polysaccharide in EPS, indicating that C8-HSL served as a crucial signaling molecule to enhance the synthesis of TB-PN. Under ribavirin stress, C8-HSL played a more pivotal role in directing the synthesis of polysaccharides, suggesting that ribavirin might interfere with or inhibit the TB-PN synthesis pathway. Correlation analysis of C4-HSL further corroborated that ribavirin stress induced alterations in the metabolic pathways of sludge microorganisms. Ribavirin stress exerted a significant interference effect on the microbial community composition, promoting the dominant proliferation of signal molecule synthesizing bacteria such as Acidovorax, drug-resistant bacteria including Acinetobacter and Delftia, along with other bacterial species that secreted biotoxic factors during the early stage of AGS culture. Nevertheless, the microbial community composition within the AGS system ultimately achieved a high degree of similarity across various conditions, suggesting that microbial diversity and community succession were pivotal factors contributing to the stability and effective pollutants removal capabilities of the AGS system.
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