GUOLuo-xiu,LIMing-gang,ZHONGWen-jie,et al.Treatment of Aged Landfill Leachate Using Pilot-scale RBC-O/A-MBR[J].China Water & Wastewater,2025,41(5):108-114.
RBC-O/A-MBR处理晚期垃圾渗滤液中试研究
- Title:
- Treatment of Aged Landfill Leachate Using Pilot-scale RBC-O/A-MBR
- Keywords:
- aged landfill leachate; partial nitrification and denitrification; rotating biological contactor; microbial community composition
- 摘要:
- 采用基于短程硝化反硝化(PND)的生物转盘(RBC)-O/A-MBR组合工艺处理晚期垃圾渗滤液,分析运行过程中各工艺单元对污染物的去除效果及微生物群落结构的变化。结果表明,通过控制DO在0.3~0.7 mg/L成功启动了短程硝化。在RBC充分利用原水BOD5的前提下按C/N=1.6向A池投加甲醇,总无机氮去除率(TINRE)可达99.02%。16S rRNA高通量测序结果表明,第121天活性污泥和RBC生物膜中的优势菌属为甲醇反硝化菌Hyphomicrobium(39.06%、16.96%),且norank_f__Anaerolineaceae(9.11%、5.48%)可能是系统中的优势氨氧化菌(AOB)。
- Abstract:
- The integrated process consisting of rotating biological contactor (RBC), O/A and MBR based on partial nitrification and denitrification (PND) was employed to treat aged landfill leachate. The pollutant removal efficiency and change in microbial community composition within each process unit during operation were evaluated. Partial nitrification was effectively initiated by maintaining DO within the range of 0.3 mg/L to 0.7 mg/L. The total inorganic nitrogen removal efficiency (TINRE) achieved 99.02% when methanol was added to anoxic tank at C/N of 1.6, under the condition that BOD5 in the influent was fully utilized by the RBC. The 16S rRNA high-throughput sequencing results indicated that one of the predominant bacterial genera in both the activated sludge and the RBC biofilm was the methanol-denitrifying bacteria Hyphomicrobium on the 121st day, accounting for 39.06% and 16.96%, respectively. Additionally, norank_f__Anaerolineaceae (9.11% in activated sludge and 5.48% in RBC biofilm) might potentially represent the dominant ammonia-oxidizing bacteria (AOB) in the system.
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