LITan,ZOUKang,YUHua-rong.Analysis of Biochemical Characteristics of Activated Sludge Based on Front?face Fluorescence[J].China Water & Wastewater,2025,41(7):117-123.
基于前表面荧光的活性污泥生化特性解析
- Title:
- Analysis of Biochemical Characteristics of Activated Sludge Based on Front?face Fluorescence
- Keywords:
- front-face fluorescence; activated sludge; nicotinamide adenine dinucleotide(NADH); biochemical characteristics; oxygen uptake rate (OUR); substrate degradation rate
- 摘要:
- 活性污泥的耗氧速率（OUR）和底物降解速率是污泥生化特性的重要参数，但其检测步骤繁琐，无法原位实时监测。基于此，采用前表面荧光激发-发射矩阵（FF-EEM）对序批式反应器（SBR）中活性污泥在一个反应周期内的荧光特性进行分析，并对比了OUR与底物降解速率的变化。结果表明，FF-EEM检测到的烟酰胺腺嘌呤二核苷酸（NADH）在稳定期和内源呼吸期与底物降解速率的相关性良好（相关系数为0.915 3）；在整个周期内，NADH与活性污泥耗氧速率有很好的相关性（相关系数为0.682 0）。因而，有望通过FF-EEM在线监测活性污泥中NADH荧光来分析其生化特性，进而指导工艺的智慧调控。
- Abstract:
- Oxygen uptake rate (OUR) and substrate degradation rate in activated sludge are critical parameters for assessing the biochemical characteristics of activated sludge. However, traditional detection methods are complex and do not allow for in situ monitoring. To address this issue, the fluorescence characteristics of activated sludge in a sequential batch reactor (SBR) over a complete cycle were analyzed using front-face fluorescence excitation-emission matrix (FF-EEM). Additionally, the variations in OUR and substrate degradation rate were examined and compared. Nicotinamide adenine dinucleotide (NADH), as detected by FF-EEM, exhibited a strong correlation with substrate degradation rate during both stable and endogenous respiratory periods (R2=0.915 3). Throughout the entire cycle, NADH also showed a significant correlation with the OUR of activated sludge (R2=0.682 0). Consequently, it is anticipated that FF-EEM can be employed to monitor NADH fluorescence in activated sludge online, thereby facilitating the analysis of biochemical characteristics and guiding the intelligent regulation of the treatment process.
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