[1]陈群,张亚,王媛媛,等.连续流AOA工艺碳氮磷去除与微生物群落结构特征[J].中国给水排水,2025,41(13):10-17.
　CHENQun,ZHANGYa,WANGYuan-yuan,et al.Characteristics of Carbon, Nitrogen, and Phosphorus Removal and Microbial Community Structure of a Continuous Flow AOA Process[J].China Water & Wastewater,2025,41(13):10-17.
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连续流AOA工艺碳氮磷去除与微生物群落结构特征

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第41卷 期数: 2025年第13期 页码: 10-17 栏目: 出版日期: 2025-07-01
Title:
Characteristics of Carbon, Nitrogen, and Phosphorus Removal and Microbial Community Structure of a Continuous Flow AOA Process
作者:
陈群1， 张亚2， 王媛媛3， 赵骥2， 邱艳玲2， 冯娟2， 王晓霞2
（1.青岛双元水务有限公司，山东 青岛 266109；2.青岛大学 环境与地理科学学院， 山东 青岛 266071；3.青岛水务集团环境能源有限公司，山东 青岛 266002）
Author(s):
CHEN　Qun1， ZHANG　Ya2， WANG　Yuan-yuan3， ZHAO　Ji2， QIU　Yan-ling2， FENG　Juan2， WANG　Xiao-xia2
（1. Qingdao Shuangyuan Water Co. Ltd.， Qingdao 266109， China； 2. School of Environmental and Geographical Sciences， Qingdao University， Qingdao 266071， China； 3. Qingdao Water Group Environmental Energy Co. Ltd.， Qingdao 266002， China）
关键词:
连续流AOA工艺;  全程硝化;  反硝化除磷;  微生物群落结构;  高通量测序
Keywords:
continuous flow AOA process;  complete nitrification;  denitrifying phosphorus removal;  microbial community structure;  high?throughput sequencing
摘要:
以模拟城市污水为处理对象，采用连续流AOA反应器接种二沉池剩余污泥，并通过调控进水COD浓度、曝气量、进水流量等探究了AOA脱氮除磷系统启动的最适条件和优化运行特性。经70 d的连续运行，TIN去除率最高可达60.93%。在厌氧段，聚磷菌（PAOs）和聚糖菌（GAOs）充分利用进水中的有机碳源，COD浓度可降至42.20 mg/L，释磷量达23.49 mg/L；在好氧段，PAOs的好氧吸磷效果较好（达20.70 mg/L），同时NH4+-N去除率稳定达到100%，实现了NH4+-N向NO3--N的完全转化；在缺氧段，PAOs和GAOs利用NO3--N进行内源短程反硝化和除磷，NO2--N积累率可达66.80%。高通量测序结果表明，聚糖菌Candidatus_Competibacter的相对丰度从0.96%增加至1.20%，聚磷菌Candidatus_Microthrix、Hyphomicrobium、Tetrasphaera的相对丰度分别从4.04%、2.51%、3.18%增加至15.36%、5.28%、5.41%，促使系统呈现出稳定的厌氧释磷、好氧吸磷、缺氧内源短程反硝化和除磷效能。
Abstract:
In this study, simulated municipal wastewater was treated utilizing a continuous flow AOA reactor inoculated with residual sludge from a secondary sedimentation tank. The optimal conditions for the initiation and operational characteristics of the AOA nitrogen and phosphorus removal system were systematically explored by modulating the influent COD concentration, aeration rate, and influent flow rate. Following 70 days of continuous operation, the total nitrogen removal efficiency achieved a maximum of 60.93%. In the anaerobic zone, the polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) utilized the organic carbon sources with an effluent COD concentration of 42.20 mg/L; at the same time, PAOs completed phosphorus release, with the highest release amount reaching 23.49 mg/L. In the aerobic zone, the aerobic phosphorus uptake by PAOs exhibited considerable effectiveness, attaining a concentration of 20.70 mg/L, while the removal efficiency of NH4+-N remained consistently at 100%, facilitating the complete conversion of NH4+-N to NO3--N. In the anoxic zone, PAOs and GAOs utilized NO3--N for endogenous short-cut denitrification and phosphorus removal, achieving a NO2--N accumulation rate of 66.80%. High?throughput sequencing analysis results revealed that the relative abundance of GAOs Candidatus_Competibacter increased from 0.96% to 1.20%. Furthermore, the abundance of PAOs Candidatus_Microthrix, Hyphomicrobium, and Tetrasphaera exhibited significant increases, rising from 4.04%, 2.51%, and 3.18% to 15.36%, 5.28%, and 5.41%, respectively. This resulted in the system demonstrating stable anaerobic phosphorus release, aerobic phosphorus uptake, anoxic endogenous short-cut denitrification, and phosphorus removal efficiency.
更新日期/Last Update: 2025-07-01