YANGJingyi,LIHongyuan,WENYuhui,et al.Screening of Heterotrophic Nitrifying Bacteria and Construction of an Enhanced Nitrogen Removal System for Mustard Tuber Wastewater[J].China Water & Wastewater,2026,42(7):65-71.
榨菜废水异养硝化菌筛选与强化脱氮系统构建
- Title:
- Screening of Heterotrophic Nitrifying Bacteria and Construction of an Enhanced Nitrogen Removal System for Mustard Tuber Wastewater
- Keywords:
- heterotrophic nitrification; enhanced nitrogen removal; mustard tuber wastewater; strain screening
- 摘要:
- 针对高盐榨菜废水脱氮系统构建困难、脱氮效能较低的问题，开展榨菜废水异养硝化强化脱氮系统构建与效能研究。首先从榨菜废水脱氮系统中分离纯化出异养硝化菌株，系统分析其生理生化特性与脱氮性能，而后制备成异养硝化纯菌液，并考察菌液投加对系统构建、脱氮效能及途径的影响。试验结果表明：从榨菜废水处理系统中分离纯化的JZ18菌株为施氏假单胞菌（Pseudomonas stutzeri），属于异养硝化-好氧反硝化菌。该菌株可在温度为15~47 ℃、pH=5~9、盐度为10~70 g/L（以NaCl计）条件下生长，对COD和NH4+-N的降解速率分别为23.96和0.90 mg/（L·h）。投加纯菌液的反应器可在20 d内构建出异养硝化强化脱氮系统，系统的异养、自养硝化速率分别为8.45、3.14 mg/（L·h），好氧、缺氧反硝化速率分别为11.46、7.28 mg/（L·h），COD、NH4+-N、TN平均去除率分别为94.65%、99.64%和98.80%。优势脱氮功能菌属包括盐单胞菌属（Halomonas）、弧菌属（Vibrio）、假单胞菌属（Pseudomonas）、栖苏打菌属（Nitrincola）、黄杆菌属（Flavobacterium）、瘤胃解蛋白质菌属（Proteiniclasticum）、叶杆菌属（Phyllobacteriaceae）以及硝酸盐还原菌属（Nitratireductor）。该系统通过异养硝化、自养硝化、好氧反硝化以及缺氧反硝化等多路径协同脱氮作用，实现高效稳定的脱氮效能。
- Abstract:
- Considering the challenges and low efficiency in the construction of nitrogen removal system for high-salt mustard tuber wastewater, this study investigated the development and performance of an enhanced heterotrophic nitrification-denitrification system for such wastewater. A heterotrophic nitrifying bacterial strain was isolated and purified from the nitrogen removal system for mustard tuber wastewater, and its physiological and biochemical properties as well as nitrogen removal performance were systematically examined. The purified bacterial solution was then introduced into the reactor to study its effects on system construction, nitrogen removal efficiency and route. The results showed that the isolated strain JZ18, identified as Pseudomonas stutzeri, was a heterotrophic nitrifying-aerobic denitrifying bacterium, which could grow under temperatures ranging from 15 ℃ to 47 ℃, pH between 5 and 9, and salinities of 10-70 g/L (calculated as NaCl). The degradation rates of COD and NH4+-N reached 23.96 mg/(L·h) and 0.90 mg/(L·h), respectively. By adding purified bacterial solution to the reactor, the enhanced heterotrophic nitrification system was constructed within 20 days. The system exhibited heterotrophic and autotrophic nitrification rates of 8.45 mg/(L·h) and 3.14 mg/(L·h), and aerobic and anoxic denitrification rates of 11.46 mg/(L·h) and 7.28 mg/(L·h), respectively. The average removal efficiencies of COD, NH4+-N and TN were 94.65%, 99.64% and 98.80%, respectively. Dominant denitrifying genera were Halomonas, Vibrio, Pseudomonas, Nitrincola, Flavobacterium, Proteiniclasticum, Phyllobacteriaceae, and Nitratireductor. The system has achieved highly efficient and stable nitrogen removal effect through the synergistic action of multiple pathways, including heterotrophic nitrification, autotrophic nitrification, aerobic denitrification and anoxic denitrification.
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