可沉微藻转化油脂潜力及PHB合成试验研究
- Title:
- Lipids Conversion Potential and PHB Synthesis of Settleable Microalgae
- Keywords:
- settleable microalgae; secondary effluent; polymer; lipids; poly-β-hydroxybutyrate (PHB); cyanobacteria
- 摘要:
- 可沉微藻在去除二级出水中氮、磷的同时，可以合成并积累多种具有高附加值的聚合物，如油脂、蛋白质、多糖、聚β-羟基丁酸酯（PHB）等。前期试验在“冲淘”压力下筛选出了沉淀率高达97%的可沉微藻，并通过一系列环境变化（通入CO2、实行光/暗循环等）刺激油脂聚积，最高可获得49.2%（细胞干质量，下同）的油脂含量。进一步强化试验显示，以增加光照强度与CO2通入量达到进一步提高可沉微藻油脂含量的做法事与愿违，反而出现油脂含量下降的现象。而可沉蓝藻具有相当的合成、积累PHB的能力，只要存在少许特异性碳源（乙酸盐）刺激，可沉蓝藻在不通入CO2的情况下即可轻松合成并积累含量高达30%左右的PHB。这种主要以无机碳源合成PHB的方法比以有机碳源合成PHB的活性污泥法更具吸引力。
- Abstract:
- Beyond removing nitrogen and phosphorous from secondary effluent, the settleable microalgae can also synthesize and accumulate such high value-added polymers as lipids, proteins, polysaccharides, poly-β-hydroxybutyrate (PHB), etc.The previous experiments efficiently enriched settleable microalgae with the settleable rate of more than 97% by applying “washout” pressure, and accumulated the highest lipids content of 49.2% (dry cell weight) by changing environmental factors like CO2, light/dark shift, etc. According to the following experiment results, increasing the light intensity and CO2 intake would not further increase the lipids content of the settleable microalgae. Instead, the lipids content decreased. However, settleable cyanobacteria had a good ability to synthesize and accumulate PHB. As long as there was a tiny specific carbon source (acetate) stimulation, cyanobacteria could easily synthesize and accumulate PHB with the content of up to 30% without CO2 input.Clearly, synthesizing PHB mainly from inorganic carbon source was more attractive than that from organic carbon source by activated sludge.
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