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[1]巩有奎,李剑慧,李美玲,等.不同碳源驯化聚糖菌反硝化及N2O释放特性[J].中国给水排水,2021,37(5):17-25.
　GONG You-kui,LI Jian-hui,LI Mei-ling,et al.Characteristics of Denitrification and N2O Emission of Acclimated Glycogen Accumulating Organisms under Different Carbon Sources[J].China Water & Wastewater,2021,37(5):17-25.

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不同碳源驯化聚糖菌反硝化及N₂O释放特性

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第37卷期数: 2021年第5期页码: 17-25 栏目: 出版日期: 2021-03-01

Title:: Characteristics of Denitrification and N₂O Emission of Acclimated Glycogen Accumulating Organisms under Different Carbon Sources

作者:: 巩有奎^1,2，李剑慧¹，李美玲¹，彭永臻²; (1.烟台职业学院建筑工程系，山东烟台264670；2.北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京100124)

Author(s):: GONG You-kui^1,2,LI Jian-hui1,LI Mei-ling¹,PENG Yong-zhen²; (1. Department of Architecture Engineering, Yantai Vocational College, Yantai 264670, China; 2. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China)

关键词:: 反硝化聚糖菌; 内源反硝化; N₂O释放; 聚-β-羟基烷酸; 胞内糖原

Keywords:: denitrifying glycogen accumulating organisms; endogenous denitrification; N₂O emission; polyhydroxyalkanoate; glycogen

摘要:: 为了确定反硝化聚糖菌（DGAOs）的脱氮性能及N₂O释放特性，采用序批式生物反应器，分别以乙酸钠和葡萄糖为碳源（反应器分别记作SBRAc和SBRGl），考察其脱氮过程中的碳源变化以及N₂O释放特性。结果表明，SBR_Ac和SBR_Gl的总氮去除率分别为（80.2±2.8）%和（63.4±3.5）%，N2O产率分别为（7.16±1.43）%和（13.35±2.46）%。以乙酸钠为碳源时，聚糖菌厌氧阶段吸收的有机物主要以胞内聚-β-羟基烷酸(PHA)形式储存；以葡萄糖为碳源时，部分有机物用于胞内糖原（Gly）的积累，PHA合成量减少。聚糖菌内源反硝化过程中，依次利用胞内PHA和Gly作为内碳源，且PHA提供电子的速率远大于Gly，导致SBR_Gl内NO₂^-大量积累、N₂O释放量增加。NO₂^-对氧化亚氮还原酶活性的抑制效应是导致聚糖菌内源反硝化过程释放N₂O的主要因素。与葡萄糖相比，乙酸钠更易被反硝化聚糖菌吸收为易利用的内碳源PHA，并降低反硝化过程中N₂O的释放量。

Abstract:: In order to investigate the denitrification efficiency and N₂O emission characteristic of denitrifying glycogen accumulating organisms (DGAOs), variation of the endogenous polymer and N₂O emission characteristic during endogenous denitrification process were explored in two separated sequencing batch reactors (SBR_Ac and SBR_Gl) using sodium acetate and glucose as carbon sources respectively. The total nitrogen removal efficiencies of SBR_Ac and SBR_Gl were (80.2±2.8)% and (63.4±3.5)%, and the N₂O yields were (7.16±1.43)% and (13.35±2.46)%, respectively. When sodium acetate was used as carbon source, organics absorbed by DGAOs in anaerobic stage was stored in the form of endogenous polyhydroxyalkanoate (PHA). When glucose was used as carbon source, part of the organics was used for intracellular glycogen (Gly) accumulation, and PHA synthesis decreased. In the endogenous denitrification process of DGAOs, PHA and Gly were successively used as endogenous carbon sources, and the rate of electron supply by PHA was much higher than that of Gly, leading to accumulation of a large amount of NO₂^- and increase of N₂O emission in SBR_Gl. The inhibitory effect of NO₂^- on activity of nitrous oxide reductase was the main factor leading to N₂O emission during the endogenous denitrification of DGAOs. Compared with glucose, sodium acetate was more easily absorbed by DGAOs as an easily used endogenous carbon source PHA, and the emission of N₂O during denitrification was reduced.

更新日期/Last Update: 2021-03-01