[1]逯南南,石近淼,褚福敏,等.水中耐热大肠菌群滤膜检测法优化[J].中国给水排水,2021,37(12):164.
　LU Nan-nan,SHI Jin-miao,CHU Fu-min,et al.Optimization of Filtration Membrane Method for Heat-resistant Coliforms Detection in Water[J].China Water & Wastewater,2021,37(12):164.
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水中耐热大肠菌群滤膜检测法优化

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第37卷 期数: 2021年第12期 页码: 164 栏目: 出版日期: 2021-06-17
Title:
Optimization of Filtration Membrane Method for Heat-resistant Coliforms Detection in Water
作者:
逯南南，石近淼，褚福敏，刘轲，赵清华，孙韶华，贾瑞宝
(山东省城市供排水水质监测中心，山东济南250021)
Author(s):
LU Nan-nan,SHI Jin-miao,CHU Fu-min,LIU Ke,ZHAO Qing-hua,SUN Shao-hua,JIA Rui-bao
(Shandong Province City Water Supply and Drainage Water Quality Monitoring Center, Jinan 250021, China)
关键词:
耐热大肠菌群; 粪大肠菌群; 滤膜法; 酶底物法
Keywords:
heat-resistant coliforms; fecal coliforms; filtration membrane method; enzyme substrate method
摘要:
耐热大肠菌群是《生活饮用水卫生标准》（GB 5749—2006）中的常规检测项目，推荐的滤膜检测法过程简单、费用低廉，被广泛应用，但存在检测结果准确性欠佳的问题。对检测试剂、耗材及培养条件等检测条件进行了优化研究，并深入探讨了湿度对耐热大肠菌群生长的影响，结果表明检测用培养基、滤膜和培养温度、湿度是影响滤膜法检测耐热大肠菌群结果的关键要素。优化方法采用商品化培养基、一次性无菌滤膜、人工气候箱培养，湿度控制在40%左右，能够有效提高检测结果的准确度。
Abstract:
Heat-resistant coliforms are a routine test item in Standards for Drinking Water Quality (GB 5749-2006). The recommended filtration membrane detection method has the advantages of simple procedure and low cost, and is widely applied. However, the accuracy of detection results of this method is not good. Detection conditions such as detection reagent, consumables and culture conditions were optimized, and influence of humidity on the growth of heat-resistant coliforms was discussed. The culture medium, filter membrane, culture temperature and culture humidity were the key factors that affected the filter membrane method results in the detection of heat-resistant coliforms. The optimized method adopted commercial culture medium, disposable aseptic filter membrane and artificial climate incubator, and controlled the humidity at about 40%, which effectively improved the accuracy of the detection results.

相似文献/References:

[1]张玉娟,窦月芹,张学胜,等.生物滞留系统对雨水径流中粪大肠菌群的去除效果[J].中国给水排水,2021,37(19):126.
　ZHANG Yu-juan,DOU Yue-qin,ZHANG Xue-sheng,et al.Removal Efficiency of Fecal Coliforms in Stormwater Runoff by Bioretention System[J].China Water & Wastewater,2021,37(12):126.

更新日期/Last Update: 2021-06-17