GUOLu,WANGXiao-jun,QIN Jia-fu,et al.Effect of Carbon Source Dosing Mode on Partial Denitrification Performance[J].China Water & Wastewater,2022,38(3):74-80.
碳源投加方式对短程反硝化性能的影响
- Title:
- Effect of Carbon Source Dosing Mode on Partial Denitrification Performance
- Keywords:
- partial denitrification; carbon source dosing mode; nitrite accumulation; microbial community
- 摘要:
- 短程反硝化是非常有前景的硝酸盐废水前处理方法，可为厌氧氨氧化提供必需的底物（NO2--N），而不同碳源投加方式会影响短程反硝化的性能。在进水NO3--N为100 mg/L、乙酸钠为碳源、碳氮比为2的条件下，探究了不同碳源投加方式（1次投加、3次投加、6次投加）对短程反硝化氮素转化特性及反应速率的影响。结果表明，分次投加碳源可以在短时间内启动高效稳定的短程反硝化，且6次投加方式条件下短程反硝化性能最优。6次投加碳源（t=0/10/20/30/40/50 min）条件下短程反硝化出水NO3--N、NO2--N平均浓度分别为7.33、60.92 mg/L，NO3--N至NO2--N的平均转化率（NTR）为86.55%，NO3--N比还原速率和NO2--N比还原速率分别为26.79、4.14 mg/（g·h）。高通量测序结果显示，拟杆菌门和变形菌门是短程反硝化系统中的优势菌门。在研究过程中，短程反硝化功能菌属Thauera丰度逐渐增加，3种投加方式下其相对丰度分别为0、14.29%、17.11%，说明与短程反硝化相关的优势菌得到富集。
- Abstract:
- Partial denitrification is a promising pretreatment method for nitrate wastewater, which can provide essential substrate (NO2--N) for Anammox. However, different carbon source addition methods will affect the performance of partial denitrification. Under the condition that influent NO3--N was 100 mg/L, sodium acetate was the carbon source, and the ratio of carbon to nitrogen was 2, the effects of different carbon source dosing modes (one time dosing, three times dosing and six times dosing) on nitrogen transformation characteristics and reaction rate of partial denitrification were investigated. The partial denitrification with high efficiency and stability could be started in a short time by multiple dosing of carbon sources, and the partial denitrification performance with dosing of carbon source in six batches was the best. When carbon source was added for six times (t=0/10/20/30/40/50 min), the average NO3--N and NO2--N in the effluent were 7.33 mg/L and 60.92 mg/L, respectively, the average nitrogen transformation rate (NTR) of NO3--N to NO2--N was 86.55%, and the specific reduction rates of NO3--N ratio and NO2--N were 26.79 mg/(g·h) and 4.14 mg/(g·h), respectively. High?throughput sequencing showed that Bacteroidetes and Proteobacteria were the dominant bacterial phyla in the partial denitrification system. During the operation, the abundance of Thauera (a functional bacterium of partial denitrification) increased gradually, and its relative abundance in the systems with three dosing modes was 0, 14.29% and 17.11%, respectively, indicating that the dominant bacteria related to partial denitrification were enriched.
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