[1]李方旭,杨伊菡,周小红,等.污水中包膜病毒离心超滤浓缩方法研究[J].中国给水排水,2022,38(7):28-32.
　LIFang-xu,YANGYi-han,ZHOUXiao-hong,et al.Concentration of Enveloped Viruses in Wastewater by Centrifugal Ultrafiltration Method[J].China Water & Wastewater,2022,38(7):28-32.
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污水中包膜病毒离心超滤浓缩方法研究

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第38卷 期数: 2022年第7期 页码: 28-32 栏目: 出版日期: 2022-04-01
Title:
Concentration of Enveloped Viruses in Wastewater by Centrifugal Ultrafiltration Method
作者:
李方旭， 杨伊菡， 周小红， 杨云锋， 刘锐平， 陆韻， 刘艳臣， 黄霞
（清华大学 环境学院，北京 100084）
Author(s):
LI　Fang-xu， YANG　Yi-han， ZHOU　Xiao-hong， YANG　Yun-feng， LIU　Rui-ping， LU　Yun， LIU　Yan-chen， HUANG　Xia
（School of Environment， Tsinghua University， Beijing 100084， China）
关键词:
包膜病毒;  污水;  超滤;  浓缩
Keywords:
enveloped virus;  wastewater;  ultrafiltration;  concentration
摘要:
针对污水中包膜病毒高效富集浓缩方法缺乏的问题，以Phi6噬菌体为模式包膜病毒，系统考察了超滤浓缩方法对污水样本中Phi6噬菌体的富集浓缩效果。结果表明，超滤筒的截留分子质量越小，对污水中Phi6噬菌体的回收率就越高，但所需浓缩时间也会增加。综合考虑回收率和耗时，推荐采用截留分子质量为10 ku的超滤筒，在4 ℃条件下3 000g正向离心28 min、1 000g反向收集5 min，浓缩倍数为110倍，回收率可达到（32±3.7）%。
Abstract:
In view of the lack of methods for efficient concentration of enveloped viruses, this paper systematically investigated the performance of centrifugal ultrafiltration method for concentration of the bacteriophage Phi6 (taking it as a model enveloped virus) in wastewater. The recovery rate of Phi6 increased with the decrease of molecular weight cut-off of the ultrafiltration cartridge, however, accompanying with the prolonged concentration time. Considering the recovery performance and time, it was recommended to select the ultrafiltration cartridge with the molecular weight cut?off of 10 ku. Under the conditions of 4 ℃, forward centrifugation of 3 000g for 28 min and backward centrifugation of 1 000g for 5 min, and 110 times of concentration ratio, the recovery rate reached (32±3.7)%.

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更新日期/Last Update: 2022-04-01