LIUZhan-hua,SONGXiu-lan,LIUYao-hui.Effect of Different Kinds of Carbon Source on PHA Synthesis and Microbial Community of Activated Sludge[J].China Water & Wastewater,2023,39(11):1-7.
碳源类型对活性污泥合成PHA和微生物群落的影响
- Title:
- Effect of Different Kinds of Carbon Source on PHA Synthesis and Microbial Community of Activated Sludge
- 关键词:
- 活性污泥; 聚羟基脂肪酸酯(PHA); 短链脂肪酸; 碳源; 微生物群落
- Keywords:
- activated sludge; polyhydroxyalkanoate (PHA); short chain fatty acid; carbon source; microbial community
- 摘要:
- 为探究过硫酸氢钾复合盐（PMS）耦合剩余污泥碱性发酵获取碳源合成聚羟基脂肪酸酯（PHA）的可行性，分别采用剩余污泥碱性（pH=10）发酵液（简称“pH10发酵液”）、PMS耦合剩余污泥碱性（pH=10）发酵液（简称“pH10+PMS发酵液”）作为碳源，研究活性污泥混合菌群在富集和合成阶段产PHA的情况以及微生物群落结构的变化。结果表明，当分别以pH10发酵液、pH10+PMS发酵液作为碳源时，富集阶段PHA的最大合成量分别为10.61%和12.22%，合成阶段PHA的累积合成量分别为42.84%和52.53%；采用不同碳源时富集产PHA的微生物群落结构存在差异性，与pH10发酵液碳源组相比，pH10+PMS发酵液碳源组中与PHA合成相关的功能菌群即副球菌属、陶厄氏菌属和甲基杆菌属的含量更高，更有利于PHA的合成，因此pH10+PMS发酵液为更适宜的碳源。
- Abstract:
- To explore the feasibility of synthesizing polyhydroxyalkanoate (PHA) using carbon source from alkaline fermentation of excess sludge coupled with potassium perbisulfate compound salts (PMS), the simulated excess sludge alkaline (pH=10) fermentation solution (pH10 fermentation solution) and simulated PMS coupled with excess sludge alkaline (pH=10) fermentation solution (pH10+PMS fermentation solution) were used as carbon sources respectively to investigate the PHA production of mixed bacteria in activated sludge and the change of microbial community composition in the enrichment and synthesis stages. When the two kinds of fermentation solutions were used as carbon sources, the maximum PHA yields in the enrichment stage were 10.61% and 12.22%, and the cumulative PHA yields in the synthesis stage were 42.84% and 52.53%, respectively. The microbial community composition in enrichment stage was different when different carbon sources were used for PHA production. Compared with pH10 fermentation solution as carbon source, the content of functional bacterial group (such as Paracoccus, Thauera and Meganema) related to PHA synthesis was higher when the carbon source was pH10+PMS fermentation solution, which was more conducive to PHA synthesis. Therefore, the pH10+PMS fermentation solution was a more suitable carbon source.
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