MA Jian-ye,RONG Hong-wei,WANG Jing-yin,et al.Microbial Community Composition and Membrane Fouling Performance of Anaerobic Ammonia Oxidation Membrane Bioreactor[J].China Water & Wastewater,2021,37(9):7-14.
MBR中厌氧氨氧化菌群落结构及膜污染性能研究
- Title:
- Microbial Community Composition and Membrane Fouling Performance of Anaerobic Ammonia Oxidation Membrane Bioreactor
- Keywords:
- anaerobic ammonia oxidation (Anammox); planktonic state; nitrogen removal; membrane fouling; microbial community composition
- 摘要:
- 采用浸没式厌氧氨氧化膜生物反应器(Amx IMBR)培养厌氧氨氧化菌，当进水氨氮与亚硝态氮浓度均为450 mg/L、氮负荷为0.6 kg/(m3·d)时，总氮去除率稳定在86%左右，氨氮、亚硝态氮去除率分别稳定在90%、97%以上；氨氮去除量 ∶亚硝态氮去除量 ∶硝态氮生成量基本为1 ∶1 ∶0.18。Amx IMBR的临界通量为8.63 L/(m2·h)，这与长期运行的结果相符。膜污染的来源主要是紧密型胞外聚合物，其中蛋白质和多糖含量分别为2.42、0.84 g/m2。运行过程中，厌氧氨氧化菌形态从红棕色颗粒污泥变成了浮游态污泥，是Amx IMBR最显著的特征之一。高通量分析结果显示，浮游态污泥中的优势菌群主要为拟杆菌门(Bacteroidetes，47.83%)、浮霉菌门(Planctomycetes，21.2%)、变形菌门(Proteobacteria，18.85%)，其中优势厌氧氨氧化菌属为Candidatus Kuenenia，相对丰度为17.08%。
- Abstract:
- Anammox bacteria were cultured in an immersed anaerobic ammonia oxidation membrane bioreactor (Amx IMBR). When NH4+-N and NO2--N in the influent were both 450 mg/L and nitrogen loading rate was 0.6 kg/(m3·d),the removal rates of TN, NH4+-N and NO2--N stably reached 86%, above 90% and 97%, respectively. In addition, the ratio of ΔNH4+-N, ΔNO2--N and ΔNO3--N was basically 1 ∶1 ∶0.18. Critical flux of the Amx IMBR was 8.63 L/(m2·h),which was consistent with the long-term operational results. Tightly bound extracellular polymeric substances were the key factors to membrane fouling, in which the content of protein and polysaccharide were 2.42 g/m2 and 0.84 g/m2, respectively. During the operation, morphology of the Anammox bacteria changed from red granular sludge to planktonic sludge, which was one of the most significant characteristics of the Amx IMBR. High-throughput sequencing results indicated that the dominant microbial species of the Amx IMBR were Bacteroidetes (47.83%), Planctomycetes (21.2%) and Proteobacteria (18.85%). Among them, the dominant Anammox functional bacterium was Candidatus Kuenenia with relative abundance of 17.08%.
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