GUIZhi-gang,HEJun-guo,ZENGXin-hui,et al.Startup and Operation Efficiency of Shortcut Nitrification and Denitrification Process in AO Reactor Induced by UVA[J].China Water & Wastewater,2024,40(11):8-12.
UVA诱导实现短程硝化反硝化工艺启动与运行效能
- Title:
- Startup and Operation Efficiency of Shortcut Nitrification and Denitrification Process in AO Reactor Induced by UVA
- Keywords:
- activated sludge; UVA; AO reactor; shortcut nitrification and denitrification; biological nitrogen removal
- 摘要:
- 利用强度为232.96 μW/ cm2的紫外线A（UVA，365 nm）照射活性污泥，研究了在连续流下实现城市污水短程硝化反硝化的可行性，考察了UVA对活性污泥中氨氧化菌（AOB）、亚硝酸盐氧化菌（NOB）、反硝化菌等活性的影响。采用连续流AO反应器进行无UVA辐照与有UVA辐照的对比，在常温以及进水COD为100 mg/L、NH4+-N为50 mg/L的条件下，运行80 d后无UVA辐照的脱氮率达到50%以上，有UVA辐照的脱氮率达到80%以上，说明试验组成功实现了城市污水的短程硝化反硝化。紫外辐照对AOB的影响不大，其活性值在8 mg/（gVSS·h）左右，而对NOB的活性影响较大，从无UVA辐照的10 mg/（gVSS·h）左右减少到2 mg/（gVSS·h）左右。这与UVA能够诱导微生物氧化应激效应机制有关，NOB因胞内缺乏相应的抗氧化酶，导致其无法在短时间内有效清除胞内的活性氧（ROS）。紫外辐照对反硝化菌活性有提高作用，活性值从8 mg/（gVSS·h）左右提升到12 mg/（gVSS·h）左右。
- Abstract:
- Ultraviolet A (UVA, 365 nm) with a strength of 232.96 μW/cm2 was used to irradiate activated sludge, the feasibility of achieving shortcut nitrification and denitrification of urban sewage was studied under continuous flow conditions,and the effects of UVA on the activity of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and denitrifying bacteria were investigated.In a continuous flow AO reactor, the effects of no UVA irradiation and UVA irradiation were compared. Under the conditions of normal temperature, influent COD of 100 mg/L and NH4+-N of 50 mg/L, the nitrogen removal rate of without UVA irradiation and with UVA irradiation reached more than 50% and over 80% respectively after 80 days of operation, indicating that the experimental group successfully realized the shortcut nitrification and denitrification.The effect of UV irradiation on AOB was very small, and the activity value was about 8 mg/(gVSS·h). While the activity of NOB was reduced from about 10 mg/(gVSS·h) without UVA irradiation to about 2 mg/(gVSS·h). It is speculated that this is related to the mechanism that UVA can induce microbial oxidative stress effects. NOB generally lacks corresponding antioxidant enzymes, resulting in the inability to effectively clear intracellular reactive oxygen species (ROS) in a short period of time. UVA irradiation increased the activity of denitrifying bacteria from 8 mg/(gVSS·h) to about 12 mg/(gVSS·h).
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