ZHUZhuo-yu,WANGJing-quan,WUHan,et al.Structure-Activity Characteristics and Spectral Analysis of Antibiotics Bound to Humic Acid[J].China Water & Wastewater,2025,41(17):49-56.
抗生素与腐殖酸结合态赋存构效特征与光能谱分析
- Title:
- Structure-Activity Characteristics and Spectral Analysis of Antibiotics Bound to Humic Acid
- 摘要:
- 天然水体中抗生素与腐殖酸（HA）的结合态赋存是有别于其自由态残留的常态化形式，但相关构效关系存在显著差异。采取三维荧光光谱分析和透析平衡法对不同结构抗生素与HA结合效能进行对比，基于模型化合物对比、紫外-可见光谱（UV-vis）、傅里叶红外光谱（FTIR）和核磁共振氢谱（1H NMR）等光能谱表征方式探究了抗生素与HA的结合位点。结果表明，抗生素与HA结合导致其荧光发生显著淬灭，四环素类抗生素对HA荧光淬灭率达到了25.4%，结合性能高于磺胺类、β-内酰胺类和氯霉素类抗生素。HA与化合物的结合在12 h后逐渐达到平衡，典型抗生素与HA发生结合作用后形成了更为稳定的配合物。进一步明确了抗生素不同官能团与HA的结合倾向，其排序为羰基>羧基>酚羟基，这为实际水环境中结合态抗生素的识别和分析提供了理论依据。
- Abstract:
- The bound state residues of antibiotics and humic acid (HA) are a common form that differs from their free-state residues in natural water, and the structure-activity relationships differ significantly. This study compared the binding efficiency of different structures of antibiotics with HA using three-dimensional fluorescence spectroscopy and the dialysis equilibrium method. Additionally, the binding sites of antibiotics with HA were explored through model compound comparisons, ultraviolet-visible (UV-vis) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance hydrogen spectroscopy (1H NMR). The results showed that the binding of antibiotics to HA significantly quenched its fluorescence. Tetracycline antibiotics had a fluorescence quenching rate of 25.4%, which was higher than that of sulfonamides, β-lactams, and chloramphenicol. The binding of HA to compounds gradually reached equilibrium after 12 hours. The interaction between typical antibiotics and HA resulted in the formation of more stable complexes. The study further clarified that the binding tendency of different functional groups on antibiotics to HA was in the order: carbonyl > carboxyl > phenolic hydroxyl. This finding provides a theoretical basis for the identification and analysis of bound?state antibiotics in aquatic environment.
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