[1]杨童,谭嘉怡,王燕,等.臭氧微纳米气泡对大肠杆菌的消毒效果及灭活机制[J].中国给水排水,2022,38(7):1-10.
　YANGTong,TANJia-yi,WANGYan,et al.Disinfection Effect and Inactivation Mechanism of Ozone Micro-nano Bubbles on Escherichia coli[J].China Water & Wastewater,2022,38(7):1-10.
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臭氧微纳米气泡对大肠杆菌的消毒效果及灭活机制

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第38卷 期数: 2022年第7期 页码: 1-10 栏目: 出版日期: 2022-04-01
Title:
Disinfection Effect and Inactivation Mechanism of Ozone Micro-nano Bubbles on Escherichia coli
作者:
杨童1， 谭嘉怡1， 王燕1， 高俊贤1， 王秉政1， 张海川1， 王硕1,2,3， 李激1,2,3
（1.江南大学 环境与土木工程学院，江苏 无锡 214122；2.江苏省厌氧生物技术重点实验室，江苏 无锡 214122；3.江苏高校水处理技术与材料协同创新中心，江苏 苏州 215009）
Author(s):
YANG　Tong1， TAN　Jia-yi1， WANG　Yan1， GAO　Jun-xian1， WANG　Bing-zheng1， ZHANG　Hai-chuan1， WANG　Shuo1，2，3， LI　Ji1，2，3
（1. School of Environmental and Civil Engineering， Jiangnan University， Wuxi 214122， China； 2. Jiangsu Key Laboratory of Anaerobic Biotechnology， Wuxi 214122， China； 3. Jiangsu University Collaborative Innovation Center of Water Treatment Technology and Material， Suzhou 215009， China）
关键词:
臭氧微纳米气泡;  臭氧微米气泡;  消毒;  大肠杆菌;  流式细胞术
Keywords:
ozone micro-nano bubble;  ozone micron bubble;  disinfection;  Escherichia coli;  flow cytometry
摘要:
臭氧消毒技术具有灭活速率高、彻底，且无二次污染等优点，但在国内污水处理厂缺乏应用，且传统曝气方式下臭氧产生的气泡大，溶解性差，传质效率低。为此，将微纳米曝气技术与臭氧氧化相结合并用于污水消毒，考察了臭氧微纳米气泡的性能及对大肠杆菌的灭活机制。结果显示，臭氧微纳米气泡的溶解性更高，曝气10 min时溶解性臭氧浓度就接近8 mg/L；微纳米气泡的爆破作用与臭氧分解过程能够协同促进?OH的产生；在实际消毒效果方面，1 mg/L臭氧微纳米气泡溶液在1 min内可去除106 CFU/mL以上的大肠杆菌，比臭氧微米气泡灭活浓度高103 CFU/mL。在0.2 mg/L腐殖酸的影响下，2.5 mg/L的臭氧微纳米气泡溶液仍能灭活107 CFU/mL大肠杆菌，比臭氧微米气泡灭活浓度高102 CFU/mL。流式细胞术分析结果表明，臭氧微纳米气泡能更快地将细胞破碎裂解，使酯酶彻底失活及DNA解旋。
Abstract:
Among the existing traditional disinfection methods in wastewater treatment plants (WWTPs), ozone (O3) inactivation has the advantages of rapid and complete inactivation rate without secondary pollution. Nevertheless, it is lack of application in domestic WWTPs at present，and the traditional aeration method produces large bubbles, poor solubility, and low mass transfer efficiency. Micro-nano bubbles (MNBs) technology can be integrated with ozone inactivation to further improve mass transfer efficiency and the overall performance of the disinfection. The results showed that the solubility of O3 micro-nano bubbles (O3-MNBs) was higher, and the dissolved O3 concentration was near 8 mg/L after aeration for 10 min. The explosion of MNBs and O3 decomposition had a synergistic mechanism to promote the production of ·OH. In the experiment of actual disinfection, 1 mg/L O3-MNBs could inactivate above 106 CFU/mL Escherichia coli (E. coli) within 1 min. In addition, under the influence of 0.2 mg/L humic acid, 2.5 mg/L O3-MNBs could still inactivate 107 CFU/mL E. coli. The results of flow cytometry analysis implied that O3-MNBs could break and lyse cells faster via thoroughgoing oxidation of esterase and DNA unwinding.
更新日期/Last Update: 2022-04-01