LIUMin,FANGKuo,WANGKai-jun.Analysis on Microbial Community Composition of AAO+FBC/MBBR System[J].China Water & Wastewater,2023,39(19):11-18.
AAO+流态化生物载体工艺的菌群结构分析
- Title:
- Analysis on Microbial Community Composition of AAO+FBC/MBBR System
- 关键词:
- 城市污水; 脱氮除磷; FBC/MBBR工艺; 菌群结构; 宏基因组分析
- Keywords:
- municipal wastewater; removal of nitrogen and phosphorus; FBC/MBBR process; community composition; metagenomic analysis
- 摘要:
- 为进一步提高脱氮除磷效率，某污水处理厂在好氧池投加填料，将生化段工艺升级为改进型AAO+流态化生物载体工艺（FBC/MBBR）。为考察工艺稳定运行后的微生物菌群结构及功能，采用宏基因组测序方法对缺氧池混合液、好氧池填料和混合液样本进行分析。结果表明，在门水平上，缺氧池混合液、好氧池填料和混合液中的优势类群均为变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和绿弯菌门（Chloroflexi）。检测到的聚磷菌（PAO）主要为Accumulibacter（0.81%~0.99%）、Tetrasphaera（0.35%~0.75%）、Mycobacterium（1.00%~1.54%）；在好氧池填料上检测到较高丰度的Nitrosomonas（2.8%）和Nitrospira（2.8%）；反硝化功能菌主要包括Dechloromonas、Flavobacterium等。此外，还量化比较分析了与硝化、反硝化、厌氧氨氧化等脱氮过程相关的功能基因。
- Abstract:
- To improve the removal efficiency of nitrogen and phosphorus, the biochemical treatment of a full-scale wastewater treatment plant was renovated into improved AAO and fluidized biological carrier process (FBC/MBBR) by adding fillers to an aerobic tank. In order to investigate the composition and function of the microbial community under stable operation, metagenomic analysis was used to further analyze the samples from anoxic tank, aerobic tank filler and aerobic tank in this wastewater treatment system. Taxonomic analysis showed that the dominant bacterial phyla were Proteobacteria, Actinobacteria and Chloroflexi. The dominant polyphosphate accumulating organism (PAO) found were Accumulibacter (0.81%-0.99%), Tetrasphaera (0.35%-0.75%) and Mycobacterium (1.00%-1.54%). The relative abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) bacteria were enriched in aerobic biofilm, and the typical AOB and NOB were Nitrosomonas (2.8%) and Nitrospira (2.8%), respectively. The denitrification functional bacteria detected mainly included Dechloromonas, Flavobacterium and so on. Otherwise, various key enzymes related to nitrification, denitrification and Anammox during nitrogen removal were annotated and quantified.
相似文献/References:
[1]张玲玲,曹洋,顾淼,等.高排放标准下城市污水深度脱氮技术研究[J].中国给水排水,2021,37(13):27.
ZHANG Ling-ling,CAO Yang,GU Miao,et al.Advanced Nitrogen Removal Technology of Municipal Wastewater under High Discharge Standard[J].China Water & Wastewater,2021,37(19):27.
[2]阮超,张延军,李胡爽,等.武汉大东湖城市污水深隧结构健康监测系统研究[J].中国给水排水,2021,37(20):126.
RUAN Chao,ZHANG Yan-jun,LI Hu-shuang,et al.Research on Structure Health Monitoring System of Dadonghu Urban Sewage Deep Tunnel in Wuhan[J].China Water & Wastewater,2021,37(19):126.
[3]黄良波,黄世浏,曹贵华,等.ABR-AO生化沉淀一体化污水处理工艺设计[J].中国给水排水,2022,38(2):54.
HUANG Liang-bo,HUANG Shi-liu,CAO Gui-hua,et al.Design of ABR-AO Biochemical Sedimentation Integrative Wastewater Treatment Process[J].China Water & Wastewater,2022,38(19):54.
[4]郑晓英,徐智,张远,等.纳米氧化铈对好氧污泥颗粒化进程的影响[J].中国给水排水,2022,38(3):21.
ZHENGXiao-ying,XUZhi,ZHANGYuan,et al.Effect of Cerium Oxide Nanoparticles on Aerobic Granulation Process[J].China Water & Wastewater,2022,38(19):21.
[5]王宏伟,张国珍,武福平,等.两级A/O生物滤池与微絮凝过滤组合处理二级出水[J].中国给水排水,2022,38(5):65.
WANGHong-wei,ZHANGGuo-zhen,WU Fu-ping,et al.Two?stage A/O Biofilter Combined with Micro-flocculation Filtration for Secondary Effluent Treatment[J].China Water & Wastewater,2022,38(19):65.
[6]崔涛,李胜,田敏,等.氧化沟型A2/O工艺脱氮除磷性能评价[J].中国给水排水,2022,38(7):75.
CUITao,LISheng,TIANMin,et al.Evaluation of Nitrogen and Phosphorus Removal Performance in an Oxidation Ditch Based A2/O Process[J].China Water & Wastewater,2022,38(19):75.
[7]曾晓岚,尹嘉豪,陈亮,等.电化学工艺处理滇池流域农业面源污水[J].中国给水排水,2022,38(13):105.
ZENGXiao-lan,YINJia-hao,CHENLiang,et al.Electrochemical Process for Treatment of Agricultural Non-point Source Sewage in Dianchi Basin[J].China Water & Wastewater,2022,38(19):105.
[8]何春求,周少奇.改良A2/O—MBR工艺仿真模拟及运行优化[J].中国给水排水,2022,38(15):90.
HEChun-qiu,ZHOUShao-qi.Simulation of Modified A2/O-MBR Process and Its Operation Optimization[J].China Water & Wastewater,2022,38(19):90.
[9]刘流,倪黄蕾,梁军,等.二沉池不同径向分布活性污泥对脱氮除磷的影响[J].中国给水排水,2022,38(15):104.
LIULiu,NIHuang-lei,LIANGJun,et al.Effect of Different Radial Distribution of Activated Sludge on Nitrogen and Phosphorus Removal in Secondary Settling Tank[J].China Water & Wastewater,2022,38(19):104.
[10]李易寰,董玉明,高芳.AAOA-MBR工艺在污水厂提标扩建中的应用[J].中国给水排水,2022,38(18):114.
LIYi-huan,DONGYu-ming,GAOFang.Application of AAOA-MBR Process in Sewage Treatment Plant Upgrading and Expansion[J].China Water & Wastewater,2022,38(19):114.