CHU Fu-min,SUN Shao-hua,LU Nan-nan,et al.Isolation, Identification and Characteristic Analysis of Chlorine-resistant Bacteria in Urban Water Supply Pipe Network[J].China Water & Wastewater,2020,36(21):42-46.
供水管网中耐氯菌的分离鉴定及特性分析
- Title:
- Isolation, Identification and Characteristic Analysis of Chlorine-resistant Bacteria in Urban Water Supply Pipe Network
- Keywords:
- water supply pipe network; chlorine-resistant bacteria; Gordonia terrae; isolation; identification
- 摘要:
- 供水管网中的耐氯菌筛查及特性分析在饮用水微生物安全评估方面具有重要意义。基于传统微生物培养技术，提出了一种供水管网耐氯菌筛选分离方法，并从某市引黄供水管网中成功分离到一株耐氯菌——土地戈登氏菌（Gordonia terrae），该菌株为条件致病菌，革兰氏阳性，好氧。该菌株的耐氯性较大肠埃希氏菌显著增强，2.5 mg/L余氯在2 h内无法将浓度为107 CFU/mL的土地戈登氏菌（Gordonia terrae）全部灭活，灭活率仅为3个对数去除率，相同消毒条件下，大肠埃希氏菌在消毒剂作用20 min后基本全部被灭活。供水管网中耐氯菌的出现对水厂现行消毒工艺提出了新的挑战。
- Abstract:
- Screening and characterization of chlorine-resistant bacteria in urban water supply pipe netwrok are of great significance in microbial safety assessment of drinking water. Based on traditional microbial culture technology, a method for screening and isolating chlorine-resistant bacteria in urban water supply pipe network was proposed, and a chlorine-resistant bacterium—Gordonia terrae, was successfully isolated from a water supply pipe network of Yellow River Diversion Project. The strain was a conditional pathogen, Gram-positive and aerobic. The chlorine resistance of this strain was significantly higher compared with that of Escherichia coli. Within 2 hours, 2.5 mg/L of residual chlorine could not inactivate all the Gordonia terrae with a concentration of 107 CFU/mL, and the inactivation rate was only 3lg. Under the same disinfection conditions, Escherichia coli was basically inactivated after 20 minutes of disinfectant action. The emergence of chlorineresistant bacteria in water supply pipe network poses a new challenge to the current disinfection process in water treatment plants.
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